FIGURE 4.

Identification of unique genes overexpressed in primary murine hepatocytes transfected with truncated β-catenin. A, an immunoblot performed on primary mouse hepatocytes transfected with Δ95 β-catenin (b-cat) and full-length (FL) β-catenin indicates expression of the appropriate proteins. B, RNA-seq analysis reveals up-regulation of several known β-catenin targets after overexpression of Δ95 β-catenin. C, RNA-seq also reveals genes uniquely expressed by Δ95 β-catenin, and the top 20 targets are shown. D, Genomatix search for common transcription factor (TF) binding sites in promoters of potential Δ95 targets reveals binding sites for many factors present at a frequency significantly above what is seen in the genome at large, suggesting one or more of these factors may be co-regulating Δ95 β-catenin targets.