FIGURE 4.

Stability of fibrils against solubilization with GdmCl. The protein fraction that resisted solubilization after 1-h incubation at room temperature has been plotted. Data were normalized to the fraction of insoluble protein after washing with 50 mm Tris-HCl,, pH 7.9, 1.5 m KCl, 1 mm EDTA, 1 mm DTT in the absence of GdmCl. WT-PABPN1 (black circles), ΔAla-PABPN1 (open circles), (+7)Ala-PABPN1 (gray circles) and the truncated variant (SUMO-ΔN114) that lacks the N-terminal domain (black squares) are shown.