FIGURE 7.
RNA binding assay of fibrillar full-length PABPN1. Filter binding assays were performed with radioactive oligo(A)-RNA to determine the apparent dissociation constants (KD) of fibrillar PABPN1 variants: WT-PABPN1 (black circles), ΔAla-PABPN1 (open circles), and (+7)Ala-PABPN1 (gray circles). For comparison, monomeric WT-PABPN1 (black squares) is shown. Data points were fitted according to Ref. 4. Dashed-dotted line, monomeric WT-PABPN1; solid line, fibrillar WT-PABPN1; dotted line, fibrillar ΔAla-PABPN1; and dashed line, (+7)Ala-PABPN1.