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. 2012 May 9;287(27):22654–22661. doi: 10.1074/jbc.M111.279091

FIGURE 2.

FIGURE 2.

Repeated TGF-β1 administration inhibits expression of osteoblast differentiation markers in hMSC. A, cells were cultured in OBM (1), OBM with a single administration of 1 ng/ml TGF-β1 (2), OBM with a triple administration of 1 ng/ml TGF-β1 (3) for 4 days after which ALP activity was measured. B–E, confluent cells were treated for 96 h with OBM (1), OBM with a single administration of 1 ng/ml TGF-β1 (2), and OBM with a triple administration of 1 ng/ml TGF-β1 (3). Repeated TGF-β1 administration inhibited the expression of RUNX2 (B), ALP (C), IGF-1 (D), and OC (E). Expression of these genes was analyzed by qRT-PCR, and their mRNA levels were normalized to that of 18 S rRNA and measured in triplicate. Values represent mean ± S.E. (n = 4). Bonferroni correction for multiple comparisons was applied. *, p < 0.001.