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. 2012 May 10;287(27):22643–22653. doi: 10.1074/jbc.M111.337949

FIGURE 7.

FIGURE 7.

A, Western blot analysis of 10 μg/ml pro-MMP-2-Myc/His incubated with 50 nm thrombin in COS-1 cells for the indicated times using anti-Myc antibody. Pro-MMP-2 was purified from the conditioned medium of PMA (10 ng/ml)-treated cells expressing pro-MMP-2-Myc/His. The line graph shows the ratio of pro-MMP-2 (monomeric or dimeric) to pro-MMP-2 (monomeric or dimeric) at 0 h. Data represent the mean ± S.D. of three experiments. The bar graph shows the ratio of activated MMP-2 to monomeric plus dimeric MMP-2. Data represent the mean ± S.D. of three experiments. IB, immunoblot. B, digestion assay of fluorescein-conjugated gelatin. MMP-2 was purified from the conditioned medium of cells expressing pro-MMP-2 treated with or without thrombin (50 nm) or p-aminophenylmercuric acetate (APMA; 0.5 mm) for 6 h. The digested substrates were analyzed as described under “Experimental Procedures.” Data represent the mean ± S.D. of five experiments.