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. Author manuscript; available in PMC: 2012 Jul 6.
Published in final edited form as: Cancer Res. 2011 Jan 24;71(6):2371–2380. doi: 10.1158/0008-5472.CAN-10-2299

Figure 3. AhR upregulation leads to increased Raf pS621 phosphorylation and decreased ERK1/2 phosphorylation.

Figure 3

Cell lysates collected 48h after RA treatment (52 h after valproic acid (V)treatment) were resolved on 7.5% gel for AhR detection and 12% gel for all the other proteins. 25 µg protein was loaded per well. For treatment, RA final concentration was 1 µM and V was 1 mM. A. AhR protein levels and pS621 Raf are increased in overexpressors nontreated and in RA and/or V treated cells. B. AhR protein is upregulated and post-transcriptional modified by RA treatment as showed 72h post treatment by Western blot. AhR appear to largely reside in the cytosol. C. Valproic acid antagonizes ERK phosphorylation.