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. Author manuscript; available in PMC: 2012 Jul 6.
Published in final edited form as: Cancer Res. 2011 Jan 24;71(6):2371–2380. doi: 10.1158/0008-5472.CAN-10-2299

Figure 5. Oct4 is instrumental in propelling differentiation.

Figure 5

A. confirms Oct4 downregulation in Oct4 knockdown cells. The results of flow cytometric analysis of paraformaldehyde fixed cells are expressed as mean fluorescence of the entire population. B. 6 h after RA treatment a significantly greater number of cells (P<0.05) expressed CD38. C. Oxidative metabolism is enhanced in Oct4 knockdowns (p= 0.03). Flow cytometric assay of live cells was performed setting the logical gate to exclude 95% of the untreated cells (B. and C.) D. Oct4 downregulation increases the percentage of cells in G0 -with no treatment p =0.01 (c vs. d) or with RA treatment p=0.015 (a vs. b) at 48h. In RA treated cells, S is significantly lower in Oct4 knockdowns. (flow cytometry of PI labeled nuclei).