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. 2012 Jul 6;7(7):e39398. doi: 10.1371/journal.pone.0039398

Figure 5. Scavenger receptor-B1 is expressed in rat cerebral arteries.

Figure 5

A. A representative real-time PCR trace showing the detection of mRNA of rat scavenger receptor-B1 (SRB1) (CT: 29.0±0.2) in posterior cerebral arteries (PCAs) relative to that of β-actin (CT: 22.9±0.2, n = 3). NTC - no template control. B. schematic representation of the smooth muscle and endothelial layers in the arterial wall that were imaged for immunohistochemical localization of SRB1 and eNOS. C. Immunohistochemical localization of eNOS and SRB1 in rat PCAs. Left panel – endothelial layer; right panel – smooth muscle layer; Blue – DAPI, nuclear stain; green – eNOS; red – SRB1 and merge – composite image of three colors. SRB1 appears to be mostly localized in the endothelial layer, partly co-localized with eNOS, and nuclear localization is evident in endothelial cells and partly in smooth muscle cells.