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. 2012 Jul 6;7(7):e39398. doi: 10.1371/journal.pone.0039398

Figure 8. NO release by IGFBP-3 is independent of CamKII activation.

Figure 8

A. Determination of NO generation in response to 4αPDD or IGFBP-3 by DAF-FM fluorescence microscopy in HMVECs. Shown were color scale for DAF-FM fluorescence and representative images of HMVECs with different treatments. UP to 40 cells were imaged per treatment in three independent experiments. B. Quantification of DAF-FM fluorescence expressed as percent of time control illustrating the effects KN93, CamKII blocker, and its negative control KN92 on NO generation by 4αPDD or IGFBP-3. NO generation by 4αPDD was significantly attenuated by KN93 (*P<0.02), but not by KN92, whereas NO generation by IGFBP-3 was not affected by either KN93 or KN92.