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. 2012 Jul 6;7(7):e39977. doi: 10.1371/journal.pone.0039977

Figure 3. FGF8 planar induction from IsO has initial tissue preferences in mesencephalon.

Figure 3

Classical FGF8 soaked bead implantation in mesencephalon induces ERK1/2 activity before earliest induction of mRNA (Mkp3) could be detected (D). Interestingly asymmetric distribution is detected during the first two hours after incubation (A, A’ and B, B’). This asymmetry is lost from 3 hours onwards after bead implantation (C, C’). In cryostat sections a mesencephalic bead implantation induced highly intense ERK1/2 activation as observed at the rostral side of the bead after 1 hour of incubation (A’). After 2 hours, ERK phosphorylation is detected caudal to the bead (B’; see small asterisk). Finally ERK activity is homogeneously distributed at both rostral and caudal cells after 4 hours (C’). Red asterisks indicate an FGF8 soaked bead; blue asterisk indicate a PBS bead. Scale bars are 0,5 mm except for A’, B’, C’ that are 50 µm, in D’ is 0,25 mm.