Figure 9. Effect of myosin II activation and Mn²⁺ on HL-60 cell migration.

(A) Immunoblotting for pRLC shows that cells migrating on FN or ICAM-1 show similar levels. For the HL-60 cells, pRLC levels appear to be dose independent on both substrates. Representative blots are shown, and relative-fold increase of pRLC presented. The data are derived from at least three experiments for each substrate. (B) RLC activation does not affect the migratory properties of HL-60 cells on FN. HL-60 cells were doubly transfected with paxillin-mCherry and RLC, RLC-A,D, or RLC-D,D with GFP. Experimental time: 4 minutes. Scale Bar  = 10 µm. (C) Migration of HL-60 cells was inhibited by Mn²⁺. Three individual cells before and after Mn²⁺ treatment were plotted. Set 1: red; set 2: green; set 3: blue. P value  = 0.012.