Figure 4. Regulation of apoptotic signaling by calcitonin and SDCP.

(A) Western blot analysis of Bcl-2 and Mcl-1 expression in osteoclasts treated with CT (10 nM), SDCP (10 µM), or both. (B) Caspase 8 and 9 activation in response to CT (10 nM) or SDCP (10 µM) stimulation. Western blot analysis revealed the presence of two fragments (p43 and p18), corresponding to the cleaved caspase 8 under the treatment of FasL (10 ng/mL). Western blot analysis of cleaved caspase 9 was shown in the right panel. Protein levels were quantified by densitometry, corrected for the sample load based on actin expression, and expressed as fold-increase or decrease relative to the control lane. Each blot is representative of at least three replicate experiments.