Figure 6. Plasma circulatory half-life of TPP1 preparations.

Tpp1(−/−) mice were administered 0.12 mg (Top) or 2 mg (Bottom) of unmodified or modified TPP1 via tail vein injection and plasma samples collected via cheek puncture at indicated times. Unmodified TPP1, open circles; OX-TPP1, filled circles, DG-TPP1, gray squares. Plasma TPP1 activity was assayed after preactivation, normalized to the percentage of the activity at the initial time point and fit to a single phase exponential decay model constraining the plateau value to 0%. Curves show a single representative animal for each condition and insets represent mean and SEM for the indicated number of animals.