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Tpp1(−/−) mice were administered unmodified enzyme or vehicle alone via tail vein injection and tissues collected after 24 hours. Tissue processing and immunostaining was conducted as described previously [33]. Top Panels show the choroid plexus and hippocampus (original magnification 4×) with inset showing hippocampal CA2 neurons (20×). Bottom Panels show cerebral cortex layer 2/3 (20× magnification) with inset showing select neurons (60×).
