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. 2012 Jul 6;7(7):e40324. doi: 10.1371/journal.pone.0040324

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Lam et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–B) Protein-protein interaction of TPM3 and GEP was validated by immunoprecipitation using anti-TPM3 antibody, followed with immunoblot detection using anti-GEP antibody in different cell lines (A) Hep3B and (B) HepG2 cells. (C) Positive correlation of GEP and TPM3 on protein level. AS: Cells suppressed for GEP by transfection with anti-sense GEP fragment; FL: Cells overexpressed for GEP by transfection with full-length (FL) GEP cDNA construct. Cells with overexpression of GEP showed elevated TPM3 levels, while suppression of GEP showed decreased TPM3.