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. 2012 Jul 6;7(7):e40312. doi: 10.1371/journal.pone.0040312

Figure 2. Lef1 expression changes over the course of the mouse estrus cycle.

Figure 2

(A) Mouse uteri were segregated based on phase of the estrus cycle (n = 3 per phase) and evaluated for Lef1 protein expression by Western blotting; GAPDH was used as a loading control. (B) Quantitative analysis of Lef1 protein expression (as measured by Image-J analysis) according to phase of the estrus cycle (average of values for all samples in a given phase, with error bars). Note the increase in Lef1 protein levels during proestrus, the time at which gland development is at its peak. (C) Quantitative analysis of cyclin D1 expression in the glandular epithelium (the number of cells positive for cyclin D1 was divided by the total number of nuclei within the glandular epithelium). Note that cyclin D1 levels peak during proestrus, coinciding with the peak in gland development and the initial increase in Lef1 levels. Error bars represent SEM.