Table 1. Tryptic peptides unique to rhumAb B identified by Mascot ETS and SIEVE, respectively, at 1% spiking level.
| # | # of trypticpeptide | # of AA | MH+ | ID byMascot | ID bySIEVE |
| 1 | HC T14 | 49 | 5056.55 | + | + |
| 2 | HC T21 | 33 | 3789.76 | + | + |
| 3 | LC T6 | 30 | 3363.50 | + | + |
| 4 | HC T11 | 29 | 3160.42 | + | + |
| 5 | HC T19 | 27 | 2971.40 | + | + |
| 6 | HC T38* | 23 | 2803.24 | + | + |
| 7 | LC T3 | 21 | 2352.17 | + | (+) |
| 8 | HC T2 | 19 | 2249.97 | + | + |
| 9 | HC T4 | 21 | 2197.12 | + | + |
| 10 | HC T1 | 20 | 1910.05 | + | + |
| 11 | HC T35* | 17 | 1901.93 | + | + |
| 12 | LC T1 | 18 | 1898.02 | + | + |
| 13 | HC T32 | 16 | 1876.90 | − | + |
| 14 | HC T15 | 14 | 1665.75 | + | + |
| 15 | HC T13** | 14 | 1424.69 | + | + |
| 16 | HC T8 | 11 | 1338.68 | + | + |
| 17 | HC T12** | 12 | 1288.64 | + | + |
| 18 | LC T7 | 12 | 1264.63 | + | + |
| 19 | HC T9 | 11 | 1247.54 | + | + |
| 20 | HC T23*glycosylated | 9 | 1173.52 | − | + |
| 21 | LC T4** | 9 | 1064.57 | + | + |
| 22 | HC T28 | 8 | 830.46 | (+) | + |
| 23 | LC T2 | 6 | 708.33 | + | + |
| 24 | LC T5 | 7 | 685.40 | − | − |
| 25 | HC T6 | 5 | 623.35 | + | + |
| 26 | HC T3 | 5 | 504.28 | − | − |
| 27 | HC T22 | 4 | 501.31 | − | − |
| 28 | LC T8 | 4 | 488.31 | − | − |
| 29 | HC T18 | 4 | 462.26 | − | − |
| 30 | HC T7 | 4 | 461.24 | − | − |
| 31 | HC T16 | 3 | 361.21 | − | − |
| 32 | HC T5 | 2 | 232.14 | − | − |
| 33 | HC T10 | 2 | 232.14 | − | − |
| 34 | HC T17 | 1 | 175.12 | − | − |
Positive identification by SIEVE is given for ratio values >2.5; positive identification by Mascot is given for ion score values >20 using a database that contains the spiking antibody sequence.
*
Tryptic peptide differs in one amino acid between rhumAb A and rhumAb B.
**
Tryptic peptide differs in two amino acids between rhumAb A and rhumAb B.
(+)These peptides gave a slightly lower score in SIEVE and Mascot respectively but still were considered as identified after manual data interpretation.