Table 2. Assignment of hits detected by differential analysis (see Figure 4) and relative quantitation of single amino acid mutations.
| # | RT [min] | SIEVE ratio value1 | MH+ | Mascot ID | Manual ID (rel. amount at 2L/100 L fermentation scale [%]) | Comment |
| 1 | 14.7 | 10 | 3440.46 | − | HC T22-23+biantGal2wFuc2 | production clone specific glycopattern differences |
| 2 | 15.7 | 11 | 2958.16 | − | HC T22+biantGal2wFuc | |
| 3 | 18.1 | 22 | 2121.95 | + | LC T14 Glu→Asp (0.4/0.5) | single point mutation |
| 4 | 22.7 | 6 | 1876.92 | + | LC T18 | unmodified tryptic peptide |
| 5 | 36.8 | 7 | 2574.29 | − | TATGVHS-LC T1 (0.7/n.d.) | production clone specific signal peptide remaining3 |
| 6 | 43.0 | 7 | 1920.97 | + | LC T1 | unmodified tryptic peptide |
| 7 | 55.1 | 312 | 1821.01 | + | HC T24 Thr→Asn (2.7/4.0) | single point mutation3 |
| 8 | 58.7 | 172 | 1803.98 | − | Not identified |
1
:Highest Sieve ratio value found for the respective peptide.
2
:biantGal2wFuc: core fucosylated, fully galactosylated complex biantennary glycostructure.
3
:for assignment of the corresponding MS/MS spectrum see Figures S2 and S3.