Skip to main content
. 2012 Jul 6;7(7):e40242. doi: 10.1371/journal.pone.0040242

Figure 6. Basic principles of the new assay system for detection of intact and real intact PTH in human samples.

Figure 6

The new detection process for real intact bioactive PTH consists of 2 steps: Firstly, any oxidized forms of oxidized PTH at position Met8 and/or position Met18 will be removed from the probe by a specific affinity chromatography column. The column contains monoclonal rat/mouse antibody (MAB) raised against the hPTH(1–34)oxidized fragments. These antibodies are able to remove any oxidized forms of PTH. In the second step the remaining non-oxidized PTH will be analyzed in conventional 2-site “sandwich” immunoassay systems. The antibody on the left (capture antibody) is bound to solid phase. The antibody on the right (label antibody) relays the signal. These antibodies must bind different sites on the PTH analyte to produce a positive result in the assay.