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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mol Microbiol. 2012 Jun 14;85(2):378–391. doi: 10.1111/j.1365-2958.2012.08118.x

Figure 1.

Figure 1

Stability effects caused by inactivation of dotL, dotM, dotN, icmS, and icmW. Total protein extracts from L. pneumophila strains grown to early stationary phase were analyzed by SDS-PAGE followed by Western blotting with antibodies specific for DotL, DotM, DotN, IcmS, and IcmW. The protein isocitrate dehydrogenase (ICDH) served as a loading control. Genotypes of the strains used are shown at the top and the antibody used for western analysis is shown at the right. Lane 1 is wild-type L. pneumophila (Lp02), lane 2 is ΔdotL ΔldsB (JV5976), lane 3 is ΔdotM ΔldsB (JV6019), lane 4 is ΔdotN ΔldsB (JV5972), lane 5 is ΔicmS (JV1962), lane 6 is ΔicmW (JV3598), lane 7 is ΔdotA (JV2064), and lane 8 is ΔldsB (JV5775).