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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cell Calcium. 2012 May 6;52(1):93–102. doi: 10.1016/j.ceca.2012.04.009

Fig. 2. The regulation of mitochondrial NAD(P)H production by increases in cytosolic calcium.

Fig. 2

Hepatocytes isolated from chow-fed rats were loaded with low levels of fura2/AM, as described previously [17, 18, 38], then stimulated with submaximal levels of phenylephrine, an α- adrenergic agonist. Agonist-evoked increases in fura-2 and NAD(P)H fluorescence intensities were monitored simultaneously [38]. NAD(P)H responses were normalized to the peak 360 nm fluorescence intensity changes obtained in the presence of rotenone plus ß-hydroxybutyrate (not shown). Data reproduced from [24] with permission.