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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cell Calcium. 2012 May 6;52(1):93–102. doi: 10.1016/j.ceca.2012.04.009

Fig. 3. Cytosolic calcium spikes stimulate a rise in mitochondrial proton motive force.

Fig. 3

Hepatocytes isolated from chow-fed rats were loaded with fura2/AM and TMREE (A) or fluorescein diacetate (B-C) then treated with submaximal hormone concentrations. Hormone-evoked increases in Ca2+ and mitochondrial membrane potential (ΔΨm) or Ca2+ and mitochondrial pH gradients (ΔpHm) were monitored simultaneously as described [17, 38]. The protonophore, FCCP (5 μM), was added in C to completely collapse mitochondrial PMF. The data in panel A are reproduced from [38] with permission.