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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cell Calcium. 2012 May 6;52(1):93–102. doi: 10.1016/j.ceca.2012.04.009

Fig 6. Lipid droplets.

Fig 6

Hepatocytes isolated from alcohol-fed rats (top row) or their pair-fed littermate controls (middle row) were maintained in primary culture for 4 hrs. Lipid droplets were labeled for 2-4 hrs with the red fluorescent fatty acid analogue Bodipy® 558/568 C12 (red pseudocolor). Cells from pair-fed controls (middle row) were also incubated with 300 μM oleic acid to induce lipid droplet formation. Cultures were fixed with 1% paraformaldehyde and then immunoreactivity for calreticulin or SERCA (kind gift from Dr. J. Lytton) was determined. Top row: calreticulin immunoreactivity in an alcoholic hepatocyte. Middle row: SERCA immunoreactivity in a lipid-loaded control hepatocyte.

Bottom row: Live hepatocytes from chow-fed rats were stained with 200 nM ER tracker (green pseudocolor) and Bodipy® 558/568 C12 (red pseudocolor), or transfected with a mitochondrial targeted glutathione biosensor Grx1-roGFP2 (kind gift from Dr. T. Dick, green pseudocolor) then incubated with the fatty acid analogue (bottom right most panel).