Glucose-regulated expression of selected proteins in human islets by Western blot. Human islets from individual donors were cultured at 5 mM (low glucose, LG) or 15 mM (high glucose, HG) glucose for 24h and protein extracts were examined by immunoblotting using specific antibodies. Representative immunoblots (A, C, E) and quantification of 3–4 replicate experiments (B, D, F) of Dicer 1 (A, B), SLC27A2 (C, D), Phospholipase Cb4 (E, F) are shown. Tubulin was used as a control for protein loading.