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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mol Microbiol. 2012 Jun 12;85(2):345–360. doi: 10.1111/j.1365-2958.2012.08117.x

Figure 1. Over-production of two putative periplasmic-binding proteins in a B. abortus hfq mutant.

Figure 1

Whole protein lysates from the parental B. abortus strain 2308, the hfq mutant strain, and the hfq complemented strain growth in brucella both to early stationary phase were separated on 15% SDS-PAGE, and the gel was stained with RAPIDstain™. Two proteins (indicated by black arrows) were over-produced in the hfq mutant compared to the parental and complemented strains, and these two proteins were excised from the gel and subjected to electrospray ionization tandem mass spectrometric analysis. The ~30 kDa protein was identified as the B. abortus 2308 protein BAB2_0612, which is an ortholog of the Agrobacterium tumefaciens protein Atu1879. The ~40 kDa protein was identified as the B. abortus 2308 protein BAB1_1794, which is an ortholog of the A. tumefaciens protein Atu2422.