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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mol Microbiol. 2012 Jun 12;85(2):345–360. doi: 10.1111/j.1365-2958.2012.08117.x

Figure 6. AbcR1 and AbcR2 perform redundant regulatory functions.

Figure 6

The 5′-untranslated regions and the first 10 codons of BAB1_0314, BAB2_0879, or BAB2_0612 were cloned in-framed with gfp into a low-copy plasmid, and abcR1, abcR2, or a DNA fragment encoding a nonsense sRNA (the abcR2 gene in reverse) were cloned into a high-copy plasmid (see Experimental procedures). E. coli strains carrying only a gfp fusion construct, or carrying both a gfp fusion construct and a sRNA-encoding construct, were grown in LB broth, and immunoblot analyses were performed on total protein lysates to detect levels of GFP or GroEL.