Figure 2.

(A) Nitric oxide production in cocaine-treated astroglial cells. The cells were seeded in 96-well plates with complete RPMI-1640 media lacking phenol red, containing 10% FBS and treated with 2 or 3 mM cocaine for 24 h. Nitric oxide was detected with Griess reagent. Data are presented as mean ± SEM (n=15, P>0.05, insignificant in comparison to control, one-way ANOVA, Dunnett’s multiple comparison test). (B) Standard curve of sodium nitrite (25 to 400 μM).