Fig. 1.

FISH mapping of maize CBM5.08 with sorghum BAC a0023D21. a A DAPI-stained image of spread pachytene chromosomes from OMAd5.60. b FITC image showing maize chromosome 5 direct labeled with Alexa-488-dUTP-KWF total maize DNA. c Rhodamine image from A455-labeled sorghum BAC FISH signals (green arrows). d Cy-5 image of centromere FISH signal (blue arrowhead) with direct-labeled CentC. e Three-color overlay of the (red), rhodamine (green), and Cy-5 (blue) images. f Straightened projection of the maize chromosome from (e) along with three other representative images and the locations of the centromere (blue arrowhead) and CBM5.08 BAC FISH signals (green bracket) are indicated. g The representative chromosome arm with BAC signal, histogram of all signals observed on the 5S arms measured, along with the resulting cytogenetic locus name (in brackets) are shown. All scale bars represent 5 μm