Figure 6. Reduction in hydrogen sulfide synthesis during acute inflammation is neutrophil dependent.

Animals received either vehicle or anti-neutrophil serum (ANS) at 24 h and 1 h prior to zymosan-induced peritonitis. Leukocyte numbers were counted by haemocytometer (A) and cell pellets were lysed for methylene blue assay (B). **P<0.01, *P<0.05 compared by one-way ANOVA and Dunnett's post-test to vehicle alone (n = 4). NaHS (-15 min), L-PAG or CHH (1 h) were also injected i.p. prior to zymosan-induced peritonitis for 4 h. Leukocyte numbers were counted by haemocytometer (C) and Gr-1^hi neutrophils determined by flow cytometry (D). **P<0.05 compared by one-way ANOVA and Dunnett's post-test to zymosan alone (n = 5). H₂S synthesis was assessed by SF5 fluorescence intensity using spectrofluorometry in peritoneal lavage and expressed as a ratio of cell numbers (E). Leukocyte SF5 fluorescence was also measured using flow cytometry (F). ***P<0.001, **P<0.01 compared by one-way ANOVA and Dunnett's post-test to zymosan alone (n = 5).