Figure 5.

Yeast Cells Dependent on Chimeric Lysyl-tRNA Synthetases Exhibit Wild-Type Growth Kinetics but Show Differential Sensitivity to Cladosporin

(A) The homology model of ScKrs1 with cladosporin docked into the ATP binding site (asterisk). Amino acids 1–220 corresponding to the tRNA-binding domain are colored black, whereas residues beyond 220, which correspond to the aminoacylation domain, are shown in color.

(B) Growth curves of yeast cells solely dependent on heterologous, chimeric lysyl-tRNA synthetases with aminoacylation domains of the indicated species.

(C) Cladosporin dose-response curves of yeast cells solely dependent on heterologous, chimeric lysyl-tRNA synthetases with aminoacylation domains of the indicated species. Data for (B) and (C) were collected using the optical density assay and are a representation of triplicate experiments. The independent data points for each experiment are shown. In (C), a nonlinear regression curve fit was performed on the means ± SD of these data.