Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jun 21;74-20(6):1059–1074. doi: 10.1016/j.neuron.2012.04.022

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 ELL & Excerpta Medica.

Open Access under CC BY 3.0 license

PMC Copyright notice

Dorsal CA1 Reference Recapitulates BLA Unit Firing Modulation by Ventral Hippocampal θ

(A and B) A principal neuron (tjx89c) was recorded along with LFPs from dorsal (CA1 str. pyramidale) and vHippocampus (subiculum str. radiatum). (A) Raw data showing tjx89c firing during an exemplary period of ventral hippocampal θ. Traces filtered for θ frequencies are overlaid. (B) Theta oscillations in the ventral subiculum are phase-locked to those in dCA1. Phase histogram representing the distribution of vSubiculum θ troughs across the dCA1 θ cycle.

(C and D) Single units modulated in phase with dCA1 θ are similarly modulated with vHippocampus θ. (C) Left: phase histogram of tjx89c spikes computed with the dCA1 reference. vSubiculum θ trough distribution was superimposed to illustrate the predicted firing phase relationship of tjx89c with vSubiculum θ rhythm (predicted phase: 135.7°). Right: distribution of tjx89c spike angles relative to vSubiculum θ, consistent with the prediction. (D) Results obtained with dCA1 θ phase as a reference accurately reflect firing modulation of neurons in phase with ventral hippocampal θ. Top: linear correlation between the predicted (LFPs) and actual phase differences of neuron modulation by dorsal and ventral hippocampal θ. Bottom: linear correlation between modulation depths calculated with the dorsal and ventral hippocampal references. Diagonal dashed lines: unity line.

(E) Anatomically confirmed recording sites in the ventral subiculum from the same experiment. The white box highlights the Neurobiotin (green) deposit made at the str. pyramidale recording site. NeuN immunoreactivity (red) was used as a panneuronal nuclear and cytoplasmic marker to delineate str. pyramidale. Calbindin immunoreactivity (light blue) highlights hippocampal layers. Colocalization with NeuN is indicated by white and pink. Inset: higher magnification of the area delineated in the main panel. Arrow: recording site in str. radiatum. SO: str. oriens; SP: str. pyramidale: SR, str. radiatum; SLM: str. lacunosum moleculare.

Scale bars: (A) time: 1 s, LFPs: 0.5 mV, units: 2 mV; (E) 1 mm, inset: 100 μm. See also Figure S9 and Table S6.