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. Author manuscript; available in PMC: 2012 Jul 9.
Published in final edited form as: Nat Neurosci. 2011 Jul 3;14(8):973–983. doi: 10.1038/nn.2857

Figure 2. CaMKIIβ regulates dendrite patterning in vivo.

Figure 2

a, Post-natal day six (P6) rat cerebellar slices transfected by a biolistics method with the CaMKIIβ RNAi or control U6 plasmid together with GFP were subjected to immunohistochemistry using the GFP antibody. Scale bar = 10 μm. b, CaMKIIβ knockdown neurons had significantly longer dendrites compared to control U6-transfected neurons (ANOVA, p < 0.0001). 199 neurons were measured. c, Schematic of in vivo electroporation approach. d, Rat pups electroporated in vivo with a U6-CaMKIIβi/CMV-GFP RNAi or control U6/CMV-GFP plasmid were sacrificed five days after electroporation and cerebella were subjected to immunohistochemistry using the GFP and Calbindin antibody. Representative control-transfected granule neurons are shown. Scale bar = 10 μm. e, Rat pups were electroporated as in (d) and representative neurons for each condition are shown. Scale bar = 10 μm. f, IGL granule neurons analyzed as in (d) were subjected to morphometric analysis. Total dendrite length was significantly increased in IGL granule neurons in CaMKIIβ knockdown animals compared to control U6 animals (ANOVA, p < 0.0001). 266 neurons were measured. g, Rat pups electroporated in vivo with the U6-CaMKIIβi/CMV-GFP RNAi or control U6/CMV-GFP plasmid were sacrificed nine days after electroporation and cerebella were analyzed as in (d). (Top) Representative cerebellar sections from each condition are shown. Scale bar = 10 μm. (Bottom) Representative IGL granule neurons for each condition are shown. Scale bar = 10 μm. Inset: Zoomed view of dendritic tips of individual neurons. Scale bar = 2.5 μm. Bracket identifies dendritic claws.