Figure 5. .

Inflammasome components in OHT astrocytes. Western blot analysis using specific antibodies detected a subset of NLRs named NLRP3 and cleaved caspase-1 predominantly in OHT astrocytes. Immunofluorescence labeling of the OHT rat retina indicated prominent localization of NLRP3 and caspase-1 (green) in GFAP-positive (red) astrocytes. Arrows show GFAP-negative cells in the RGC layer exhibiting no detectable immunolabeling for NLRP3 or caspase-1. Blue corresponds to nuclear DAPI labeling. Data represent three independent sets of analyses with different samples. Scale bar 100 μm.