Figure 5. .

Inhibition of TNF-α expression in the presence of morphine (1 mg/kg; IP) 7 days post injury. Rats were divided into two groups: ocular-hypertensive group (n = 4); and morphine-treated ocular-hypertensive group (n = 6). Seven days post hypertonic-saline injection into the limbal veins, animals were euthanized and retina extracts (15 μg retina protein) were analyzed by Western blotting using selective anti-TNF-α primary antibodies. The signal was captured using appropriate secondary antibodies (HRP-conjugated; dilution 1:3000), enhanced chemiluminescent reagent, and the Biorad Versadoc imaging system. Data shown are representative of 4 to 6 independent experiments. Data are expressed as mean ± SE. *P < 0.05; n = 4–6.