Figure 7. .

Inhibition of activated, cleaved caspase-3 (17 kDa) in the presence of morphine (1 mg/kg; IP). Rats were divided into two groups: ocular-hypertensive group (n = 4); and morphine-treated ocular-hypertensive group (n = 4). Seven days post hypertonic-saline injection in limbal veins, animals were euthanized and retina extracts (15 μg retina protein) were analyzed by Western blotting using selective anti-caspase-3 primary antibodies. The signal was captured using appropriate secondary antibodies (HRP-conjugated; dilution 1:3000), enhanced chemiluminescent reagent, and the Biorad Versadoc imaging system. Data shown are representative of four independent experiments. Data are expressed as mean ±SE. *P < 0.05; n = 4.