FIG. 2. Apoptotic mediators in the mitochondrial pathway in the gut epithelium of mice subjected to MRSA pneumonia.
Expression of pro-apoptotic mediators Bid (A) and Bax (B) were both increased in septic animals compared to shams. Expression of anti-apoptotic protein Bcl-xL (C) was also increased in septic animals compared to shams while Bcl-2 (D) was unchanged. Expression data comes from experiments with 5–7 mice/group. Protein densities were normalized to β-actin and are reported as fold difference from sham mice (assigned the value of 1 in arbitrary units). Representative western blots from 2 out of the 5–7 mice in each group are shown for each mediator.