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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Alcohol. 2011 Sep 15;45(8):785–793. doi: 10.1016/j.alcohol.2011.07.005

Figure 1.

Figure 1

Effects of ethanol metabolism on the expression of Cdc25C and Ser 216-phosphorylated Cdc25C. VA-13 cells were cultured in the presence or absence of 25 mM ethanol for 4 days, the cells were lysed, and analyzed by immunoblot for total and Ser 216-phosphorylated Cdc25C. Densitometry indicated approximately a 1.5-fold increase in Cdc25C and approximately a 10-fold increase in Ser 216-phosphorylated Cdc25C in cells cultured in the presence of ethanol, when normalized with b-actin. The blot is representative of at least three experiments. Error bars indicate the standard error of the mean *P ≤ 0.05. C: control, E: ethanol treated