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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Alcohol. 2011 Sep 15;45(8):785–793. doi: 10.1016/j.alcohol.2011.07.005

Figure 3.

Figure 3

Effects of ethanol metabolism on the expression of Thr 68-phosphorylated Chk2. VA-13 cells were cultured in the presence or absence of 25 mM ethanol and the alcohol dehydrogenase inhibitor 4-methyl pyrazole for 4 days. The cells were lysed and analyzed by immunoblot for the presence of the active, phosphorylated form of Chk2. Densitometry revealed approximately a 10-fold increase in phosphorylated Chk2 in the cells cultured in the presence of ethanol when normalized to b-actin. No increase in phosphorylated Chk2 was detected in lysates from cells cultured in the presence of 4-methyl pyrazole or 4-methyl pyrazole and ethanol. The blot is representative of at least three experiments. Error bars indicate the standard error of the mean *P ≤ 0.05. C: control, E: ethanol, P: 4-methyl pyrazole, PE: ethanol and 4-methyl pyrazole.