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RT-PCR analysis of Cdc25c and Chk2 transcripts. VA-13 cells were cultured in the presence or absence of 25 mM ethanol for three days. RNA was isolated, reverse transcribed, and analyzed by RT-PCR. Analysis revealed that ethanol metabolism slightly decreased the abundance of Cdc25c transcripts and increased Chk2 transcripts 2-fold. Data is representative of at least three experiments. Error bars indicate the standard error of the mean *P ≤ 0.05.
