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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Alcohol. 2011 Sep 15;45(8):785–793. doi: 10.1016/j.alcohol.2011.07.005

Figure 6.

Figure 6

Cell cycle analysis. Cells were cultured in the presence or absence of 25 mM ethanol and 1 mM caffeine for 4 days, stained with Vindelov’s reagent, and analyzed by flow cytometry. Approximately 4% of untreated controls and cells cultured in the presence of caffeine were in the G2/M transition of the cell cycle. Whereas, approximately 33% of the cells cultured in the presence of ethanol were in G2/M. Inclusion of caffeine in the ethanol containing media dramatically reduced the percentage of cells in G2/M to approximately 12%.