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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Nutr Food Res. 2011 Dec 7;56(3):454–465. doi: 10.1002/mnfr.201100270

Figure 3.

Figure 3

Suppression of STAT3 activation and NF-κB and STAT3-dependent gene expression by turmeric. (A) Turmeric suppresses STAT3 activation in a dose-dependent manner in cancer cells. U266, Panc28, HT29, MDA-MB-231, HCT-116, and KBM-5 cells were incubated with indicated concentrations of turmeric for 12 h. Whole cell extracts were prepared and assayed for STAT3 activation by Western blot analysis. (B) Turmeric suppresses expression of gene products involved in tumor cell survival, proliferation, and metastasis. Cells were treated with indicated concentrations of turmeric for 24 h. Whole cell protein extracts were prepared, separated by electrophoresis, and then transferred to the nitrocellulose membrane. The membrane was sliced according to molecular weight to probe with different antibodies. After stripping, the membrane was reprobed with β-actin to verify equal protein loading. Turmeric up-regulate the expression of Bax. Cells were treated with indicated concentrations of turmeric for 24 h, and whole cell protein extract was analyzed by western blotting using antibody against Bax.