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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Nutr Food Res. 2011 Dec 7;56(3):454–465. doi: 10.1002/mnfr.201100270

Figure 7.

Figure 7

Suppression of RANKL-induced osteoclastogenesis by tumeric. (A) Turmeric suppresses RANKL-induced NF-κB activation. RAW 264.7 cells (1×106 cells/well) were incubated either alone or in the presence of turmeric (0, 5, 25, 50, and 70 μg/mL) for 12 h and treated with RANKL (10 nM) for 30 min. Nuclear extracts were prepared and assayed for NF-κB activation by EMSA. Values below the EMSA gel indicate percent growth inhibition (left panel). Bands were quantitated using a Storm 820 phosphorimager equipped with ImageQuant software (Amersham). Data represent the mean 7SD of three measurements (right panel). *p<0.05; **p<0.01, versus control. (B) Turmeric suppresses RANKL-induced osteoclastogenesis. RAW 264.7 cells (1×104 cells/well) were incubated either alone or in the presence of RANKL (5 nM) and the indicated concentration of turmeric for 5 days and then stained for TRAP expression. TRAP-positive cells were photographed (original magnification, ×100). Data represent the mean 7SD of three measurements. *p<0.05; **p<0.01, versus control.