Figure 4.

Genomic structure of the ∼109 kb LCR2q21.1 subunits of 97.7% DNA sequence identity, in which NAHR sites were mapped in patient 2. (A) The NAHR site regions in patient 2 were narrowed to a 181 bp interval by sequencing the long-range PCR products obtained with the forward primer F specific to the proximal subunit and the reverse primer R, specific to the distal subunit (arrows depicting primers are not shown to scale). (B) Schematic representation of the GC content of the ∼109 kb LCR2q21.1 subunits with NAHR sites mapping to over 60% GC-rich fragment.