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. 2012 May 9;21(15):3408–3420. doi: 10.1093/hmg/dds173

Figure 4.

Figure 4.

Skeletal abnormalities in Hus1neo/neoAtm−/− embryos and adult mice. (A) Shown is a representative image of Alizarin red–Alcian blue-stained skulls from E18.5 littermates of the indicated genotypes. Dotted lines outline the region where the skull plates have yet to fill in and fuse. The arrowhead indicates the fenestrations present in the Hus1neo/neoAtm−/− skull. (B) Skulls were prepared from 6-week-old littermates of the indicated genotypes and photographed. The arrowhead indicates the fenestrations present in the parietal bone. The arrow indicates abnormal sutures of the mutant. Lower panels: higher magnification view of sutures in the Hus1neo/neoAtm−/− (left) and Hus1+/neoAtm−/− (right) skulls. (C) A representative image of the skulls from 6-week-old littermates of the indicated genotypes is shown. The outline was generated in Adobe Photoshop to illustrate the doming of the skull. (D and E) 3D reconstructions based on micro-CT data for forelimbs (D) or hindlimbs (E) from Hus1neo/neoAtm−/− and Hus1+/neoAtm−/− mice, and the bar graphs of the corresponding measurement data (n=3 per genotype). Red arrowheads point to the mesophalanx of digit V. The relative length of mesophalanx V in both forelimbs and hindlimbs was significantly shorter in Hus1neo/neoAtm−/− mice when compared with all other genotypes (*P< 0.001, Student's t-test).