Figure 5.
Neutralizing antibody (NAb) responses as measured in A3R5 cells. NAbs were assessed against a panel of tier 1 and tier 2 reference strains of Env.IMC.LucR viruses in the A3R5 assay. Plasma (RV144) and serum (Vax003) samples are the same as those described in Figure 1. Top, Positive response rates (frequency of positive results at ≥1:20 plasma dilution) against each virus (listed on the x-axis). Because of limited sample volumes, 2 viruses (427299 and CM244) were not assayed at visit 5 for Vax003 (nt, not tested). Middle, Box plots of NAb titers against each virus (7 viruses for RV144 and 5 viruses for Vax003). For the box plots, 25% of values lie below the box, 25% lie above the box, and 50% lie below the horizontal line (the median) inside the box. Vertical lines above the box extend to a distance 50% greater than the height of the box; points beyond this are unusually high values (outliers). Bottom, Magnitude-breadth (M-B) curves of NAbs against the 5 viruses that were common to the assays performed for RV144 and Vax003 (C1080, C3347, R2184, CM235, and 644039). Neutralization titer is shown on the x-axis; the fraction of viruses neutralized is shown on the y-axis. Dashed lines represent subject-specific responses. Solid lines represent group averages. Abbreviation: ID50, 50% inhibitory dose.