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. 2012 Jul 9;7(7):e40437. doi: 10.1371/journal.pone.0040437

Figure 6. Immediate-early response of NR4A1 mRNA and protein levels to TRH in GH4C1 cells.

Figure 6

A) Incubation with 1μM TRH led to a remarkable increase of the endogenous NR4A1 mRNA level with a peak within 1 hr (51-fold) and then an immediate reduction in 120 mins in GH4C1 cells. B) The TRH-induced increase of the NR4A1 mRNA level was observed in a dose-dependent manner. The minimum effect was observed with 0.1 nM TRH and then reached a plateau at 10 nM. C) Western blot analysis in GH4C1 cells demonstrated that incubation with 100 nM TRH led to a significant increase of NR4A1 protein (NR4A1) within 2 h, and NR4A1 was then degradated in 4 h, while the level of GAPDH was not altered.