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. 2012 Jul 9;7(7):e40620. doi: 10.1371/journal.pone.0040620

Figure 4. Effect of RASFib on the expression of IL15R α, β and γ chains on cocultured B cells.

Figure 4

B cells cocultured with RASFib upregulate IL-15 α, β and γ chain expression. A. Real-time quantitative analysis of IL-15R mRNA expression in B cells cocultured with RASFib for 24, 36 or 48 h. Shown is fold of induction referred to expression of IL-15R α, β and γ chain in freshly isolated B cells. Each bar represents the mean and SD of 15 subjects. B. Expression of IL-15R α, β and γ chain on the surface of non-permeabilized B cells as determined by flow cytometry. Shown is expression on freshly isolated B cells, on B cells cocultured with RASFib for 3 days and on B cells cocultured with RASFib for 6 days. Each bar represents the mean and SD of 15 subjects. *p<0.05 vs freshly isolated B cells (day 0); † p<0.05 vs B cells cocultured for 3 dys with RASFib (day 3). C. Representative flow cytometry histograms showing the expression of IL-15R α, β and γ chains on freshly isolated B cells and on B cells cocultured with RASFib for 3 or 6 days.