Figure 3. Influence of ion composition and iron chelators on cell volume.

Histograms of cell volume distribution as assessed by flow cytometry after one week of cold storage in different solutions. Storage in cell culture medium induced marked cell swelling (A), storage in chloride-rich solutions containing iron chelators slight swelling (B, C). Cell swelling in chloride-rich basic solutions was as pronounced as in cell culture medium, irrespective of sodium concentration (D), substitution of chloride by lactobionate induced marked cell shrinkage (B, C). Cell swelling was inhibited by the addition of iron chelators (E, F; basic solution 3 vs. solution 3 w/o adenosine, solution 3 vs. basic solution 3+ adenosine), but not influenced by addition of adenosine (E, F).