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. 2012 Jul 9;7(7):e40475. doi: 10.1371/journal.pone.0040475

Figure 1. DAZAP2 gene expression in multiple myeloma cell lines KM3, MM.1S, OPM-2, and ARH-77.

Figure 1

(A) DAZAP2 gene expression in normal condition. (B) DAZAP2 gene expression after treatment with 5-aza-2′-deoxycytidine. The total RNA was isolated from multiple myeloma cells and RT-PCR was performed with the specific primers to detect the gene expression level of DAZAP2. The PCR products were ran on 1.5% agarose gel. β-actin was used for normalization and verification of sample loading. The length of amplification products for DAZAP2 is 400 bp and for β-actin is 260 bp. M:100 bp DNA ladder plus marker; AZA: 5-aza-2′-deoxycytidine; (−): without treatment of 5-aza-2′-deoxycytidine as control; (+): with treatment of 5-aza-2′-deoxycytidine. Each value represents mean ± S.D. of three independent experiments.