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. 2012 Jul 9;7(7):e40469. doi: 10.1371/journal.pone.0040469

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Anand et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: Phase contrast microscopic image showing development of cellular aggregates (thin arrow) and ‘duct–like’ (bold arrow) structures in BuMECs grown in attached collagen Type I matrix for four days; B: Phase contrast microscopic image of BuMECs grown on plastic substratum for four days shows no such morphological changes; C: Phase contrast microscopic image (low magnification) showing development of cellular aggregates (thin arrow) and ‘duct–like’ (bold arrow) structures in BuMECs grown in attached collagen Type I matrix for four days; D: Fluorescent image of acini-like cellular aggregate and duct- like structure in monolayer of BuMECs grown on attached collagen Type I matrix which is evident from the propidium iodide stained nucleus of the cells forming the structures (insert image show the phase contrast image of the field). Acini-like structure (arrow) constitutes a large aggregate of PI stained nuclei and duct-like structure (arrow head) showing a clear arrangement of PI stained nuclei along the sides of duct-like structure suggesting the formation of wall and lumen. Bars, A and B 100 µm, C and D 500 µm Results represent images from two independent experiments.